What if… you could join an organization that creates, resources, and builds life sciences companies that invent breakthrough technologies to transform human health and sustainability?

Company Summary:
Quotient Therapeutics, Inc. is a privately held, early-stage company developing a novel genomics platform and therapeutics for diseases with a large unmet need.

Quotient Therapeutics, Inc. was founded by Flagship Pioneering, an innovative enterprise that conceives, creates, resources, and grows first-in-category life sciences companies. Flagship Pioneering has created over 100 groundbreaking companies over the past twenty years, all of which are pioneering novel and proprietary biological, industrial, and engineering approaches to solve major needs in human health and sustainability. These companies include Moderna (MRNA) Generate Biomedicines, Sana Biotechnology (SANA), Tessera Therapeutics, Evelo Biosciences (EVLO), Indigo Agriculture, Seres Therapeutics (MCRB), and Syros Pharmaceuticals (SYRS). To date, Flagship has deployed over $2.5 billion in capital toward the founding and growth of its pioneering companies alongside more than $19 billion of follow-on investments from other institutions. In 2021, Flagship Pioneering was ranked 12th globally on Fortune’s “Change the World” list, an annual ranking of companies that have made a positive social and environmental impact through activities that are part of their core business strategies.

Position Summary:

We are seeking a high motivated scientist to execute target validation experiments using in vitro model systems aimed to validate targets identified from Quotient platforms. 

Responsibilities:

• Tissue Dissociation and Cell Isolation: Lead and perform tissue dissociation and primary cell isolation to obtain high-quality single-cell suspensions for downstream applications, ensuring consistency and viability.
• Flow Cytometry (FACS) and Cell Sorting: Design, execute, and analyze flow cytometry experiments for cell phenotyping and functional analysis; conduct cell sorting to isolate specific immune cell populations with high purity and viability.
• CRISPR and Assay Development: Design and implement CRISPR-based genome editing in human primary immune cells; develop, optimize, and validate in vitro and ex vivo assays, including overexpression, knockdown, and knockout studies, as well as cytokine profiling and functional assays.
• Laboratory Techniques: Perform and apply laboratory techniques such as ELISA, qPCR, Western blotting, and primary cell culture, ensuring precision, reproducibility, and high-quality outcomes.
• Data Analysis and Reporting: Collect, analyze, and interpret scientific data accurately; maintain precise experimental records and contribute to the preparation of research articles, reports, and presentations.
• Leadership and Collaboration: Lead projects while collaborating with cross-functional teams to integrate results into broader research initiatives; assist in designing and implementing research protocols. Engage in regular team meetings to discuss progress, challenges, and future plans. Provide mentorship and technical guidance to junior team members to support their growth and ensure project success.

Qualifications:

• PhD in Immunology, Cell Biology, Molecular Biology, or a related discipline, with 1–3 years of industry experience (role level adjusted based on experience).
• Extensive hands-on experience in tissue dissociation, flow cytometry (FACS), and cell sorting, with proven ability to isolate and characterize immune cell populations from primary tissues.
• Solid foundation in immunology, with direct experience working with primary immune cells (T cells, B cells, macrophages) and conducting functional assays to evaluate immune responses.
• Skilled in developing and optimizing immunological assays for drug target validation, cytokine profiling, and immune function analysis.
• Strong expertise in experimental design, troubleshooting, and data interpretation, particularly related to immune system function and cellular responses.
• Proficient in genetic manipulation techniques (e.g., knockout, knockdown, and overexpression) and their application in immunological research.
• Experience with RNA-seq, CRISPR, lentivirus, and AAV transduction is highly desirable.
• Excellent data management and analysis skills, with a focus on maintaining meticulous experimental records.
• Strong communicator, both written and verbal, with the ability to clearly convey complex immunological concepts to cross-functional teams.
• Thrive in collaborative, fast-paced environments with exceptional attention to detail and reproducibility in experimental work.
• Familiarity with Good Laboratory Practice (GLP), industrial workflows, and proficiency in using Electronic Laboratory Notebooks (ELN).

 

Location: Cambridge, MA 

About Flagship

Flagship Pioneering is a bioplatform innovation company that invents and builds platform companies, each with the potential for multiple products that transform human health or sustainability. Since its launch in 2000, Flagship has originated and fostered more than 100 scientific ventures, resulting in more than $90 billion in aggregate value. Many of the companies Flagship has founded have addressed humanity’s most urgent challenges: vaccinating billions of people against COVID-19, curing intractable diseases, improving human health, preempting illness, and feeding the world by improving the resiliency and sustainability of agriculture.  Flagship has been recognized twice on FORTUNE’s “Change the World” list, an annual ranking of companies that have made a positive social and environmental impact through activities that are part of their core business strategies, and has been twice named to Fast Company’s annual list of the World’s Most Innovative Companies. Learn more about Flagship at www.flagshippioneering.com.

Flagship Pioneering and our ecosystem companies are committed to equal employment opportunity regardless of race, color, ancestry, religion, sex, national origin, sexual orientation, age, citizenship, marital status, disability, gender identity or Veteran status.

At Flagship, we recognize there is no perfect candidate. If you have some of the experience listed above but not all, please apply anyway. Experience comes in many forms, skills are transferable, and passion goes a long way. We are dedicated to building diverse and inclusive teams and look forward to learning more about your unique background.

Recruitment & Staffing Agencies: Flagship Pioneering and its affiliated Flagship Lab companies (collectively, “FSP”) do not accept unsolicited resumes from any source other than candidates. The submission of unsolicited resumes by recruitment or staffing agencies to FSP or its employees is strictly prohibited unless contacted directly by Flagship Pioneering’s internal Talent Acquisition team. Any resume submitted by an agency in the absence of a signed agreement will automatically become the property of FSP, and FSP will not owe any referral or other fees with respect thereto.